ABSTRACT Paulownia tomentosa (Thunb.) Steud. is a fast-growing, multifunctional tree species of high economic and ecological value; however, large-scale propagation remains limited by variability in conventional vegetative methods. This study developed and optimised an in vitro micropropagation protocol for P. tomentosa through systematic evaluation of initiation, shoot proliferation, rooting, and acclimatisation stages. Nodal explants were successfully established using a 10% NaOCl sterilisation protocol with an 80% survival rate on ½ MS medium supplemented with 1.0 mg/L BAP. During the proliferation stage, three concentrations of 6-benzylaminopurine (BAP; 0.2, 0.5, and 1.0 mg/L) were evaluated on MS medium. The highest multiplication coefficient (3.0, with 3–4 shoots per explant and very low callus formation) was achieved on MS medium with 0.5 mg/L BAP. At 0.2 mg/L BAP, shoot development was weak; at 1.0 mg/L BAP, excessive callus formation suppressed morphogenesis. Rooting experiments demonstrated that ½ MS medium containing 1.0 mg/L indole-3-butyric acid (IBA) and 0.1 mg/L naphthaleneacetic acid (NAA) was optimal, yielding 100% rooting frequency with a mean of 5–6 roots per microshoot (length: 4–5 cm). Rooted plantlets were successfully acclimatised in cocopeat substrate and transferred to greenhouse conditions with high survival rates. The developed protocol provides a reliable and reproducible basis for mass production of genetically uniform P. tomentosa planting material suited to Azerbaijani environmental conditions.