ABSTRACT Silver nanoparticles (AgNPs) were synthesised using aqueous extracts and homogenates of Melissa officinalis L. (Lamiaceae), a medicinally important plant species distributed across all regions of Azerbaijan. This study contributes to botanical pharmacology by characterising the phytochemical mediators in M. officinalis responsible for nanoparticle biosynthesis, with direct relevance to plant secondary metabolite research. The effects of infusion time, extract concentration, and stabilisation time on the size, shape, and morphology of the synthesised AgNPs were examined. UV-Vis spectroscopy confirmed AgNP formation through a surface plasmon resonance band in the 300– 450 nm range. Scanning electron microscopy (SEM) revealed predominantly spherical AgNPs from aqueous extracts, with a size range of 9.5–80 nm; homogenate-derived particles were rod-shaped (15–45 nm). X-ray diffraction (XRD) analysis confirmed the crystalline nature of AgNPs, with an XRDcalculated mean crystallite size of 14 nm — this measure reflects the coherent scattering domain size, which is distinct from the physical particle size range observed by SEM. Fourier transform infrared spectroscopy (FTIR) identified the hydroxyl (OH) and carbonyl (CO) functional groups of the M. officinalis extract as the principal reducing and stabilising agents. The green synthesis route is rapid, low-cost, and environmentally friendly, suggesting strong potential for biomedical applications of these botanically derived nanoparticles.