Abstract. In this reasearch, genotyping was conducted on 86 accessions of wild barley (Hordeum
vulgare L. subsp. spontaneum C.Koch) and 85 accessions of cultivated barley (Hordeum vulgare L.
subsp. vulgare) collected from 20 different regions in Azerbaijan. Amplicon sequencing technology
was employed, revealing 255 specific single-nucleotide polymorphism (SNP) markers of a polymorphic
nature. Out of the total options, 66% were transitions, and 34% were transversions. For the
studied barley collection, the Genetic Diversity Index (GDI) and Polymorphism Information Content
(PIC) were calculated as 0.347 and 0.280, respectively. Notably, the 6H chromosome exhibited the
highest number of SNP markers, whereas the 7H chromosome displayed the fewest. Utilizing cluster
analysis, the accessions were categorized into three main groups. Remarkably, the study successfully
differentiated between genotypes of wild and cultivated barley. Employing the STRUCTURE
program, the number of subpopulations (K) ranged from 2 to 5. The model with K = 4 was found to
be the most consistent with the collection’s structure. The SNP markers acquired through multiplex
amplicon sequencing demonstrate robust utility for diverse assessments and analyses within various
collections. They prove suitable for exploring genetic variability and relationships, both in barley
overall and in H. vulgare and H. spontaneum individually.

EXPLORING GENETIC DIVERSITY IN AZERBAIJANI BARLEY COLLECTION THROUGH AMPLICON SEQUENCING

JOURNAL ARTICLE published 2023 in Acta Botanica Caucasica

https://doi.org/10.30546/abc.2023.2.1.10.43.